Volume 19, Issue 1 (Vol.19, No.1, Spring 2023)
irje 2023, 19(1): 73-87 |
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Fallah T, Elikaei A, Mansour Ghanaie R, Karimi A, Sedighi I, Tariverdi M, et al . Detection of Circulating Rotavirus Genotypes in Hospitalized Children ≤5 Years of Age with Acute Gastroenteritis. irje 2023; 19 (1) :73-87
URL: http://irje.tums.ac.ir/article-1-7238-en.html
URL: http://irje.tums.ac.ir/article-1-7238-en.html
Tina Fallah1 
, Ameneh Elikaei2 , Roxana Mansour Ghanaie * 3, Abdollah Karimi4 , Iraj Sedighi5 , Marjan Tariverdi6 , Arezu Amirali1 , Tayebe Nazari4 , Negin Nahanmoghadam7 , Alireza Nateghian8 , Seyed Hamidreza Monavari9 , Seyed Mohsen Zahraei10 , Sussan Mahmoudi11 , Masoud Alebouyeh12
, Ameneh Elikaei2 , Roxana Mansour Ghanaie * 3, Abdollah Karimi4 , Iraj Sedighi5 , Marjan Tariverdi6 , Arezu Amirali1 , Tayebe Nazari4 , Negin Nahanmoghadam7 , Alireza Nateghian8 , Seyed Hamidreza Monavari9 , Seyed Mohsen Zahraei10 , Sussan Mahmoudi11 , Masoud Alebouyeh12
1- Msc. in Microbiology, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- PhD in Microbiology, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
3- Subspecialist in Pediatric Infectious Diseases, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,ghanaieroxana@gmail.com
4- Subspecialist in Pediatric Infectious Diseases, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
6- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
7- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Bouali Hospital Children’s Hospital, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
8- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Ali Asghar Children’s hospital, Iran University of Medical Sciences, Tehran, Iran
9- PhD in Medical Virology, Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
10- Medical Specialist in Infectious Diseases, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran
11- Medical specialist in Pediatrics, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran
12- PhD in Medical Bacteriology, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- PhD in Microbiology, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
3- Subspecialist in Pediatric Infectious Diseases, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
4- Subspecialist in Pediatric Infectious Diseases, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
6- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
7- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Bouali Hospital Children’s Hospital, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
8- Subspecialist in Pediatric Infectious Diseases, Department of Pediatrics, Ali Asghar Children’s hospital, Iran University of Medical Sciences, Tehran, Iran
9- PhD in Medical Virology, Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
10- Medical Specialist in Infectious Diseases, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran
11- Medical specialist in Pediatrics, Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran
12- PhD in Medical Bacteriology, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract: (424 Views)
Background and Objectives: Identification of rotavirus genotypes in children is clinically important. This study aimed to determine the spectrum of rotavirus genotypes and assess their correlation with demographic variables and clinical manifestations in hospitalized children.
Methods: To determine rotavirus genotypes, rotavirus positive stool samples of symptomatic children were included in the study between December 2019 and March 2020. RNA extraction and cDNA synthesis for VP7 and VP4 genes were performed following standard protocols. Genotypes were determined using specific primers. Validation of results was done through sequencing and bioinformatic analysis. Data were statistically analyzed using SPSS version 20 and GraphPad version 9.5.0.
Results: Among the infected patients, three genotypes emerged as dominant in the studied population. The study demonstrated a significant correlation between genotype frequency and seasonal variations (p-value=0.0077), as well as between genotypes, hospitalization, and severity of diarrhea. While significantly more types of rotavirus group A were identified with increasing age, no correlation was observed between the genotypes and gender (p-value=0.473). Furthermore, there was no significant association between genotype, dehydration rates, and the presence or absence of fever.
Conclusion: This study revealed a relatively high diversity of rotavirus genotypes in children. The findings suggest the need for further research to validate the identified correlations between certain genotypes and age groups, seasonal variations, clinical symptoms, and the efficacy of available vaccines.
Methods: To determine rotavirus genotypes, rotavirus positive stool samples of symptomatic children were included in the study between December 2019 and March 2020. RNA extraction and cDNA synthesis for VP7 and VP4 genes were performed following standard protocols. Genotypes were determined using specific primers. Validation of results was done through sequencing and bioinformatic analysis. Data were statistically analyzed using SPSS version 20 and GraphPad version 9.5.0.
Results: Among the infected patients, three genotypes emerged as dominant in the studied population. The study demonstrated a significant correlation between genotype frequency and seasonal variations (p-value=0.0077), as well as between genotypes, hospitalization, and severity of diarrhea. While significantly more types of rotavirus group A were identified with increasing age, no correlation was observed between the genotypes and gender (p-value=0.473). Furthermore, there was no significant association between genotype, dehydration rates, and the presence or absence of fever.
Conclusion: This study revealed a relatively high diversity of rotavirus genotypes in children. The findings suggest the need for further research to validate the identified correlations between certain genotypes and age groups, seasonal variations, clinical symptoms, and the efficacy of available vaccines.
Type of Study: Research |
Subject:
Epidemiology
Received: 2023/05/3 | Accepted: 2023/08/28 | Published: 2023/06/10
Received: 2023/05/3 | Accepted: 2023/08/28 | Published: 2023/06/10
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